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What it means: E. coli is a fecal indicator bacterium. Its presence in treated water is an acute health violation, meaning the system must notify customers within 24 hours. Detection requires immediate corrective action — typically a boil water advisory, investigation of contamination source, and system flushing.

Health effects: Most E. coli strains cause diarrheal illness. Some strains (e.g., O157:H7) produce toxins causing severe bloody diarrhea, hemolytic uremic syndrome (HUS — a form of kidney failure), and death — especially in children under 5 and the elderly. Even non-pathogenic strains in drinking water indicate that disease-causing organisms may also be present.

Limit: Zero (one confirmed positive sample = acute MCL violation)

What it means: The 2013 RTCR (effective 2016) replaced the original TCR. It triggers automatic E. coli testing whenever total coliform is found. A single E. coli-positive sample is an acute MCL violation. It also requires systems exceeding 5% total coliform-positive samples to conduct a formal assessment and correct any deficiencies, helping prevent contamination before it escalates.

Health effects: Total coliform bacteria indicate that the disinfection barrier has been breached or fecal contamination is entering the system. E. coli specifically indicates fecal contamination and the possible presence of disease-causing pathogens (norovirus, Salmonella, Shigella, Campylobacter). Illness can be severe in elderly, young children, and immunocompromised individuals.

Limit: Zero E. coli; ≤5% of monthly samples total coliform-positive